To characterize the direct role of −31CBS in chromatin domain organization and TAL1 transcription, we employed CRISPR-Cas9 to delete the core CTCF motif of the −31CBS in the TAL1-expressed erythroleukemia K562 and T-ALL Jurkat cells (Supplementary Figure S1A). The gene discussed is CTCF; the disease is erythroleukemia.