Here, we report four major findings: (i) we determined the frequencies of Abs against gAChR in SSc patients with GI manifestations, (ii) the mean levels of anti-gAChRα3 Abs and VEGF were significantly higher in the SSc with GI manifestations group than in the SSc without GI manifestations group, and (iii) endostatin was significantly higher in SSc patients with gAChR Abs than in SSc patients without gAChR Abs. The gene discussed is COL18A1; the disease is systemic sclerosis.