VEGF-A mRNA and protein were also detected by RT-PCR and ELISA, respectively, in AsPc-1, Capan-1, Capan-2, Dan-G, and Panc-1 human ductal pancreatic carcinoma cell lines, whereas VEGFR-1 expression was observed only in three of these cell lines (AsPc-1, Dan-G, and Panc-1) [188]. Here, FLT1 is linked to pancreatic ductal adenocarcinoma.