Methylamine, N,N-dimethyl (compound 1) and 3-penten, 2-one (compound 4) were downregulated, whereas RI1101 (compound 3); 3-heptanone, 6-methyl (compound 6); RI1227 (compound 7); RI1237 (compound 8); RI1291 (compound 10); benzaldehyde (compound 12); β-farnesene (compound 13); α-farnesene (compound 14); texanol isomer (compound 16); and 5,9-undecadien-2-ol, 6,10-dimethyl (compound 17) were upregulated in St3gal4-KO male mice, which represented the anxiety and depression model. The gene discussed is ST3GAL4; the disease is depressive symptom measurement.