In the OHE group, there were no significant changes in the mRNA and protein expression of NF-κB, TNF-α, IL-1, and IL-10 (Figures 6(a)–6(h)); however, there were significant differences between the expression levels in the OME and OHE groups (Figures 6(a)–6(h)). Here, NFKB1 is linked to otitis media with effusion.