ACTR2 and hyperinsulinemic hypoglycemia, familial, 4: For example, SIM has been applied to study spreading behavior and changes in cytoskeletal rearrangement in platelets with cytoskeletal protein deficiency; such as ARPC1-deficiency, where reduced actin-related protein 2/3 complex (Arp2/3) led to aberrant platelet spreading (66) and Wiskott-Aldrich syndrome protein (WASp) deficient platelets from patients and WASp knockout mice resulted in reduced actin nodule formation (65).