JAG1 and androgenetic alopecia: To achieve synchronized anagen development over the back skin area, 50 mice were depilated at the dorsal back area, and randomly divided into five groups with the following topical treatments in the same volume: control (30% ethanol); testosterone to induce androgenetic alopecia (negative control; 0.5% testosterone (Patel et al., 2014); Jagged1 (0.5% testosterone + 1 mg/ml Jagged1); EGF (0.5% testosterone + 30 μg/ml EGF); and Jagged1 + EGF (0.5% testosterone + 1 mg/ml Jagged1 + 30 μg/ml EGF).