In addition, the results of wound-healing assays and transwell chamber invasion assays also demonstrated that endogenous knockout of Siah1 enhanced migratory ability of SW480 and HCT116 cells, as indicated by an increase in migratory speed and number of CRC cells (Fig. 5a, b; transwell: SW480-GV248/SW480-Siah1-shRNA: P < 0.001; HCT116-GV248/HCT116-Siah1-shRNA: P < 0.0001). This evidence concerns the gene SIAH1 and colorectal carcinoma.