In the present study, we not only found that GSS protected against sepsis-induced ALI but also revealed that the above protective effects of GSS are achieved through inhibition of LPS-induced Myd88/NF-κB/TNF-α/IL-6 signaling activation instead of GEF-H1/NF-κB pathway in lung microvascular ECs. The gene discussed is TNF; the disease is Sepsis.