IL33 and Cowden disease: Therefore, this study detected the expression levels of ST2 protein on macrophages in MLN and IL-33 in intestinal tissue to analyze whether IL-33 and ST2 proteins were significantly changed in the TNBS-induced CD model, and further judged whether TsKaSPI and TsAdSPI could alleviate intestinal inflammation by affecting the IL-33/ST2 pathway.