A role of Klf5 acetylation in basal to luminal differentiation is further supported by findings from the organoid formation assay2,7,9, where interruption of Klf5 acetylation disrupted the typical basal to luminal organization of organoids, led to cyst-less organoids (Fig. 5b) with fewer and deranged basal cells and more luminal cells (Figs. 3h and 5c), and interfered with the differentiation-inducing function of DHT (Fig. 5b). This evidence concerns the gene KLF5 and cyst.