In line with our previous work (Terenzio et al., 2014a), we chose ES-MNs, which express endogenous TrkB and p75NTR, and a stably transfected line of mouse neuroblastoma N2A cells overexpressing FLAG-TrkB (hereafter N2A-FLAG-TrkB) (Chen et al., 2005), as our cellular models. Here, NGFR is linked to neuroblastoma.