INS and leukemia: Briefly, we generated bar-coded expression mutated open reading frame (ORF) clones by a HiTMMoB approach [43], and then tested the effects of mutated ORFs in IL-3-dependent Ba/F3 cells (a sensitive leukemia cell line, frequently used in drug screening) and EGF- and insulin-dependent MCF10A cells (a non-tumorigenic breast epithelial cell line) in parallel using a lentiviral approach, with wild-type counterparts as well as negative and positive experimental controls.