TSC2 and lung adenocarcinoma: The relative transcript level of ERα (ESR1) was significantly higher in 621–101 cells (CT = 30.7) relative to lung adenocarcinoma A549 cells (CT = 36.4) (p < 0.001; Fig 7A), although TSC2-reexpression (621–103 cells) did not affect ESR1 expression.