We used Cyttel‐CTC and im‐FISH (immune‐fluorescence in situ hybridization) methods to detect CTCs and to investigate the expression of EMT markers and ULBP1 on CTCs.28, 29, 30 Moreover, we investigated the relationship between the expression of EMT markers and ULBP1 in vitro in gastric cancer cell lines. The gene discussed is ULBP1; the disease is gastric cancer.