In this study, we illuminated the intracellular microbiota in CD14+CD11c+ macrophages from the intestinal lamina propria using the 16S rRNA gene amplicon sequencing method to identify bacteria that are specifically present in intestinal CD14+CD11c+ macrophages of Crohn’s disease patients, and compared bacterial flora between CD14+CD11c+CD163low and CD14+CD11c+CD163high macrophages, that have opposite inflammatory functions, to investigate whether there is a difference in bacterial flora. The gene discussed is ITGAX; the disease is Crohn disease.