Three colon cancer cell lines, each with a different Wnt pathway status, were used: RKO (intact Wnt signaling pathway), HCT116βm (WT APC, stabilized β-catenin due to Ser45 deletion)20, and DLD1 cells (APC truncation at amino acid 1452, WT β-catenin, see Fig. 1a). This evidence concerns the gene APC and malignant colon neoplasm.