siRNA candidate genes that failed to meet these criteria (WASF1, EPN3, PAK1, and AMPH1) showed no evidence of a reduction in viral trans-infection when MDDCs were transfected with individual siRNAs, nor were we able to detect a specific knockdown in protein expression, suggesting that the previously observed reduction in viral trans-infection maybe due to off-target effects of those specific siRNAs. This evidence concerns the gene PAK1 and infection.