In addition, the role of MAG2 in the formation of the cyst wall of the Pru strain was analyzed by preparing in vitro cysts of the Pru, ΔMAG2, and MAG2-COMP strains; in vitro cysts generated from these strains also revealed no distinguishing differences in the cyst morphologies of the Pru and ΔMAG2 strains (Fig. S5). The gene discussed is COMP; the disease is cyst.