Besides, the expression levels of Wnt-3a (Fig. 4d) and β-catenin (Fig. 4e) proteins, the marker protein of Wnt/β-catenin signaling pathway, were also obviously promoted, which implied the Wnt/β-catenin signaling pathway was activated by zinc in osteosarcoma cells. This evidence concerns the gene WNT3A and osteosarcoma.