We assume that it is difficult to completely avoid ADE in Ab-based experiments, but EDII-cEDIII may assist in the development of an improved recombinant Ag against DENV infection, because the ADE of DENV infection during the initial stage of virus infection was effectively inhibited by anti-EDII-cEDIII Ab compared with anti-cEDIII Ab. This evidence concerns the gene RENBP and viral infectious disease.