ALPL and Miyoshi myopathy: Then, in order to investigate the effects induced by EVs in hMSCs during osteogenic differentiation, we analyzed the mRNA levels of the early osteogenic differentiation markers, i.e., Osterix, ALPL, Col1A1, and Osteocalcin (OCN), in hMSCs treated for 10 days with EVs from MM cell lines.