To confirm previous observations of the effect of LASP1 knockdown on AKT1 phosphorylation [22,24,34], we performed experiments with MDAMB-231 and MCF-7 breast cancer cells, stably transfected with doxycycline-inducible LASP1-specific shRNA, transiently transfected with different LASP1-specific siRNAs. Here, AKT1 is linked to breast carcinoma.