found that long-term use of Indomethacin could induce the expression of PD-1 and PD-L2, and inhibit the secretion of TNF-alpha and IFN-gamma through TRIF/NF-kappa B axis and JAK/STAT3 axis in a dose-dependent manner in vivo and in vitro, and further promote intrahepatic recurrence and extrahepatic distant metastasis of hepatocellular carcinoma. This evidence concerns the gene TNF and hepatocellular carcinoma.