To the best of our knowledge, this is the first report to elaborate the physicochemical characterization and anti-invasive effects of Cudrania tricuspidata root (CTR)-GNPs synthesized from the root extracts of Maclura tricuspidata via the inhibition of MMP-2/MMP-9 and PLD activity in human glioblastoma cells. Here, GPLD1 is linked to glioblastoma.