The neuronal markers that we validated here for GABAergic (GAD67+) and dopaminergic (tyrosine hydroxylase+) neurons, have been used to document imbalanced signaling associated with learning and memory deficits in NF1 [15, 38, 64] and with other cognitive and motor impairments in Huntington’s disease [39]. The gene discussed is GAD1; the disease is Huntington disease.