KIF9 and Hydrocephalus: Obtained Kif9−16/−16 mice did not exhibit overt abnormalities including hydrocephalus that is often observed when the motility of ependymal cilia is impaired.28, 29 We confirmed that KIF9 was depleted in Kif9−16/−16 male testis and spermatozoa with Western blotting (Figure 2E) and in the null spermatozoa with immunofluorescence (Supplemental Figure S2A).