EIF5A2 and hepatocellular carcinoma: Obviously, the luciferase activity of wild-type (wt) EIF5A2 3'-UTR was decreased by increased miR-9 and was improved by reduced miR-9, while mutant (mt) EIF5A2 3'-UTR was not influenced (P < 0.05, Figure 4C).Transient transfection of miR-9 mimic in HCC cells significantly reduced EIF5A2 protein expression (Figure 4D).