EMD and osteosarcoma: In this paper we compared the nuclear phenotype of osteosarcoma cells with increasing aggressiveness [37,38] to normal osteoblasts and deepened the relationship between expression changes of pivotal nuclear envelope (NE) components, as A- and B-type lamins and emerin, in osteoblasts and osteosarcoma cell lines and their potential malignancy by confocal microscopy, biochemical and RT-PCR analyses.