Comparing morphological nucleoskeletal changes and the immunoexpression data among osteoblasts and osteosarcoma cell lines (Figure 1 and Figure 7), we found a clear association between higher lamin B1 expression levels and nuclear dysmorphisms in osteoblastic cell lines; particularly SaOS2 cells, showing the higher variability of alterations, i.e., donut-shaped or fragmented nuclei, or NEs with blebs, septa and folds, exhibited high values of lamin B1 immunoexpression (Figure 7A,C). The gene discussed is LMNB1; the disease is osteosarcoma.