Comparison of CFU percentages between 2D-monolayer and 3D cell culture model revealed that after 24 hrs p.i. in the 3D tissue model, ADH and INT rates reached levels equal to those observed in the 2D-monolayer infections at 4 hrs p.i., irrespective of the C. jejuni strain background (Fig 5A and 5B). This evidence concerns the gene AVP and infection.