Hence, we examined the effect of poly‐G ODN on the JAK/STAT1 pathway activated by IFN‐α or IFN‐β using western blotting and flow cytometry to exclude the possibility of direct inhibition of JAK/STAT1 phosphorylation in cancer cells, and clearly determine the specificity of ODN to IFN‐γ. The gene discussed is IFNG; the disease is cancer.