To investigate the relationship between exercise-induced myocardial ischemia–hypoxia and the expression patterns of autophagy-associated proteins, C-2R BG and immunohistochemical staining of the four autophagy-associated proteins (Beclin1, LC3, Cathepsin D, and p62) in adjacent slices were analyzed (Fig. 2). This evidence concerns the gene MAP1LC3A and myocardial ischemia.