ESR1 and carcinoma: Similarly, Leide and colleagues reported median age of diagnosis as 41 years.17 A hospital based study on breast cancer patients from Apulia, Italy supports our findings as it reported ductal invasive type, higher cytohistological tumor grade, negative ER and PgR status in the BRCA1 associated carcinomas.14 A possibility cannot be excluded that patients found negative for variants in the BRCA1 have variations in the BRCA2.