To determine whether the pattern of H3.3 deposition is altered in ATRX-mutant NB, we performed CUT&RUN39–41 on six NB cell lines divided into three groups: MYCN-amplified cells (SKNBE2 and IMR32), ATRX-mutant cells (SKNMM and CHLA90) and MYCN non-amplified/ATRX wild-type cells (LAN6 and SKNFI). Here, MYCN is linked to neuroblastoma.