We used eight neuroblastoma cell lines, including three MYCN-amplified lines (IMR32, SKNBE2, NB-5) or moderately elevated levels of MYCN (NBL-S) (Supplementary Data 2), two lines (SKNMM and CHLA90) produce ATRX with an in-frame deletion (ATRXIFD) and are diploid for MYCN (Fig. 2a), one osteosarcoma cell line with no detectable expression of ATRX (U2OS) and three lines as controls. The gene discussed is MYCN; the disease is osteosarcoma.