To determine whether DS37001789 had an anti-hypertrophic effect in TAC-induced mice after 4 weeks of administration, we measured the mRNA expression of regulator of calcineurin 1 (Rcan-1), which is a target gene of the calcineurin/NFAT pathway, and the mRNA expression of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP), which are sensitive markers of heart failure and hypertrophy (Figure 6). This evidence concerns the gene NPPB and heart failure.