Because reconstitution of AR nuclear localization and AR activity are common mechanisms of resistance to neoadjuvant intense ADT10, we examined nuclear AR expression using immunohistochemistry in treatment-resistant tumor foci in whole-mount RP tissues, as well as anti-PTEN and anti-ERG immunohistochemistry (Fig. 1g; Supplementary Fig. 3b). Here, ERG is linked to neoplasm.