To evaluate the impact of GLUT1 expression on iCCA progression, two cholangiocarcinoma cell lines, HuCCT1 and RBE, were stably transfected with lentiviral vector encoding short hairpin GLUT1 (sh GLUT1) or lentiviral vector encoding wild-type GLUT1. The gene discussed is SLC2A1; the disease is infantile convulsions and choreoathetosis.