In order to determine whether PERK and PAM are essential for angiogenesis in glioblastoma, we performed the standard tube formation assay where we subjected primary human umbillical vein endothelial cells (HUVECs) with conditioned media from non-target and PERK or PAM knockdown LN229 and LN308 cells treated under hypoxia and allowed them to form tubes on growth factor-reduced matrigel within 24 h. Here, EIF2AK3 is linked to glioblastoma.