We first performed a univariate analysis of the clinicopathological characteristics of NSCLC contained CHCHD2 and HIF-1a expression, according to the result of the univariate analysis, factors contained CHCHD2 (P = 0.000) and HIF-1α (P = 0.000) expression, tumor size (P = 0.012), grade of differentiation (P = 0.001), TNM stage (P = 0.000) and lymph node metastasis (P = 0.000) showed significantly higher hazard rations for a poor prognosis (Table 3). This evidence concerns the gene HIF1A and metastatic malignant neoplasm in the lymph nodes.