iTRAQ technology has been applied to muchresearch: To identify prognostic biomarkers for gastric cancer [17]; to reveal potential novel biomarkers for the early diagnosis of acute myocardial infarction within three hours [18]; to identified SAA and ACTB as potential biomarkers in patients with severe hand, foot, and mouth disease (HFMD) [19], to discover glioblastoma serum markers [20], and so on. Here, ACTB is linked to hand, foot and mouth disease.