KRAS and melanoma: Extracted DNA was then subjected to further analysis by Therascreen PCR for the mutations detectable by the following kits: Therascreen EGFR RGQ PCR kit for NSCLC samples, Therascreen KRAS RGQ PCR kit, Therascreen BRAF RGQ PCR kit for mCRC samples, and Therascreen BRAF RGQ PCR kit for melanoma samples.