Extracted DNA was then subjected to further analysis by Therascreen PCR for the mutations detectable by the following kits: Therascreen EGFR RGQ PCR kit for NSCLC samples, Therascreen KRAS RGQ PCR kit, Therascreen BRAF RGQ PCR kit for mCRC samples, and Therascreen BRAF RGQ PCR kit for melanoma samples. The gene discussed is BRAF; the disease is melanoma.