GFAP and infection: Whereas immunofluorescence analysis in Figure 2 clearly demonstrated infection of astrocytes and neurons, they also revealed that only a fraction of both cell types was positive for RABV P. To quantify the ratio of infected and non-infected neurons and astrocytes, 3D object segmentation and counting was performed for NeuN- (Figure 3a,b) or GFAP (Figure 3d,e)-positive cells.