Our results found that SIX3 is hypermethylated in glioma, SIX3 promoter contains high levels of H3K27me3 in glioblastoma tissues, knockdown of SUZ12, a core subunit of PRC2 that catalyzes H3K27me3, fails to effectively rescue the level of SIX3 (Fig. S11), suggesting that DNA hypermethylation, but not H3K27me3, is responsible for epigenetic silencing of SIX3 in glioma. This evidence concerns the gene SIX3 and central nervous system cancer.