To test the importance of IQGAP1 in metastasis in vivo, we first created stable IQGAP1 knockdowns in MA2 melanoma cells using miR30-based shRNAs (sh1 and sh6), along with control cells expressing shRNA against firefly luciferase (shFF) (Fig. 1A,B). The gene discussed is IQGAP1; the disease is melanoma.