To examine the relevance of IQGAP1 in metastasis in the context of a different cancer type, we also produced two clonal knockouts in LM2 breast cancer cells (an in-vivo-selected, highly metastatic derivative of MDA-MB-231)24, using the same two sgRNAs as above (Fig. 3A). The gene discussed is IQGAP1; the disease is cancer.