Then, we detected CNN3 protein in 10 cell lines, that included non-cancer cell lines (IMR-90, 293T, 293), cervical cancer cell lines (SiHa, Hela, CaSki), ovarian cancer cell lines (A2780, SKOV3, Caov3) and an osteosarcoma cell line (U2os). The gene discussed is CNN3; the disease is osteosarcoma.