To induce an abortion mouse model, pregnant BALB/c females, which had been mated with C57BL/6 males, were administrated intraperitoneally with anti-PDL1 blocking monoclonal antibody (mAb) or IgG (as a nonspecific control) at a dosage of 250 μg on E5.5 and E8.5, respectively. The gene discussed is CD274; the disease is abortion.