Then real-time PCR and ELISA assays were performed, the results of which indicated that STAT3 knockdown alleviated the gankyrin-mediated increase in CCL24 mRNA expression in ccRCC cells and CCL24 concentration in conditional medium of ccRCC cells (Figs. 3f and 5e; Supplementary Fig. S5a). This evidence concerns the gene STAT3 and nonpapillary renal cell carcinoma.