Validation experiments based on enzyme-linked immunosorbent assays (ELISAs) confirmed that the amount of CCL24 secreted into the CM from gankyrin-overexpressing ccRCC cells was significantly higher than that secreted in the CM from control cells (Fig. 3d), whereas a lower CCL24 level was detected in the CM from gankyrin-knockdown ccRCC cells (Fig. 3e). The gene discussed is CCL24; the disease is nonpapillary renal cell carcinoma.