In our previous studies on second-trimester MS-AFP and APOs, the AUROC was 0.686, 0.717 and 0.611 for preterm birth, preeclampsia and SGA, respectively; women with second-trimester MS-AFP ≥ 2.5 MoM had increased risks (OR, 95% CI) of preterm birth (4.10, 2.44~6.88), preeclampsia (3.95, 2.23~6.99) and SGA (3.45, 1.91~6.21) [22]. The gene discussed is AFP; the disease is preeclampsia.