Constitutive NF-κB activation was observed in 67% of colorectal cancer cell lines [24], and we also confirmed that some colon cancer cells such as DLD-1, HT-29, and LS174T display much higher p65 activity compared to normal intestinal cells (HIEC6) based on reporter assays (Figure S3, Supplementary Materials). This evidence concerns the gene NFKB1 and malignant colon neoplasm.