To identify whether TLR2 or TLR4 is responsible for the silencing of immunosuppressive Siglec ligands, we analyzed changes in disialyl Lewisa or sialyl 6-sulfo Lewisx glycan expression by knocking down TLR2 or TLR4 by applying specific shRNA for TLR2 or TLR4 in colon cancer cells. The gene discussed is TLR4; the disease is colonic neoplasm.