In cell lines, compared to the immortalized cervical epithelial cells (H8 cells), SULF2 mRNA was upregulated significantly in the cervical cancer cell lines HeLa (5.26±0.46), SiHa (4.68±0.56), Ca-Ski (3.47±0.36), C33A (3.01±0.25), and C-4I (2.48±0.29), with the highest expression found in HeLa cells (all P<0.05, Figure 1B). The gene discussed is SULF2; the disease is cervical cancer.